The Inhibitory Effects of Juglanin on Adipogenesis in 3T3-L1 Adipocytes
| Autor: | Guang Qian Wang, Wenzhou Xu, Heyuan Wang, Bing Wu, Xuefei Jin, Kun Wang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pharmacology chemistry.chemical_classification Chemistry Pharmaceutical Science AMPK Peroxisome proliferator-activated receptor Lipid metabolism 3T3-L1 Metabolism disorder Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Adipogenesis 030220 oncology & carcinogenesis Lipid droplet Drug Discovery Lipogenesis |
| Zdroj: | Drug Design, Development and Therapy. 14:5349-5357 |
| ISSN: | 1177-8881 |
| Popis: | Introduction Deregulation of adipogenesis plays an important role in obesity and other metabolism disorders. PPAR, C/EBP and SREBP1c are key transcriptional factors involved in adipogenesis and lipogenesis. Juglanin is a natural compound belonging to flavonoids, and it has been reported that juglanin has a potent inhibitory effect on inflammation and certain type of cancers. However, the effects of juglanin in adipogenesis have not been reported before. Materials and Methods 3T3-L1 preadipocytes were incubated with differentiation induction medium in the presence or absence of 0.5, 2.5, or 5 µM juglanin for an 8-day differentiation period. The lipid droplets accumulated in the cytoplasm were monitored by Oil Red O staining on days 0, 2, 5, and 8. The regulatory effects of juglanin on adipogenesis-related genes and proteins were investigated by real-time polymerase chain reaction and Western blot analysis. Results Juglanin significantly decreased lipid accumulation in differentiated adipocytes. Our findings show that juglanin reduced the expression of C/EBPα, C/EBPβ, and SREBP-1c without affecting PPARα or PPARγ expression. Additionally, juglanin increased the activation of the SIRT1/AMPK signaling pathway through the phosphorylation of AMPKα. Finally, we performed an AMPK inhibitor experiment, which revealed that the inhibitory effects of juglanin on adipogenesis are mediated through AMPK. Discussion Juglanin can prevent adipogenesis by suppressing lipid accumulation and the differentiation of preadipocytes. The mechanism of juglanin regulating adipogenesis requires further investigation. Future clinical study in vivo could shed more light on its implication in modulating obesity and metabolic disorders. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|