Occurrence of Estrogen Receptor α in Bovine Placentomes Throughout Mid and Late Gestation and at Parturition1

Autor:	Ursula Teichmann, Rudolf Leiser, Hubert Thole, Christina Wirth, Bernd Hoffmann, Klaus Failing, Gerhard Schuler
Rok vydání:	2002
Předmět:	medicine.medical_specialty Pregnancy Stromal cell Late gestation medicine.drug_class Estrogen receptor Trophoblast Cell Biology General Medicine Biology medicine.disease Monoclonal antibody medicine.anatomical_structure Endocrinology Reproductive Medicine Stroma Internal medicine Placenta medicine
Zdroj:	Biology of Reproduction. 66:976-982
ISSN:	1529-7268 0006-3363
DOI:	10.1095/biolreprod66.4.976
Popis:	The bovine placenta produces estrogens from the first trimester until the end of its life span. However, with the exception of the immediate prepartal and intrapartal phases, in which an involvement of placental estrogens has been suggested for the preparation of parturition, their function has not been elucidated yet. To test for a role of placental estrogens as local factors regulating placental growth and differentiation, placentomes from cows that were pregnant for 150, 220, 240, and 270 days, and parturient cows (3 animals per group) were screened immunohistochemically for the expression of estrogen receptor α (ERα). Indirect immunoperoxidase staining methods were applied using primary monoclonal antibodies (pmAbs) directed against the C-terminus (AER311, HT277) or the N-terminus (AER314, 1D5) of the ERα molecule. Both types of pmAbs identified ERα in stromal cells and capillary pericytes of the maternal caruncular septae. Using pmAb 1D5, the mean percentage of ERα-positive caruncular stroma...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::fd81bb3fab7dd32d732592ebbb171cd2 https://doi.org/10.1095/biolreprod66.4.976 Zobrazit plný text záznamu