THE CONTRIBUTION OF H2O2 PRODUCED BY ASPERGILLUS NIGER IN VAT BLUE DYE DISCOLORATION: ENHANCEMENT BY A STATISTICAL OPTIMIZATION METHODOLOGY

Autor:	J. Lira-Pérez, J. Melendez-Estrada, B.J. González-de Jesus, R. Rodriguéz-Vazquéz, M.E. Hidalgo-Lara
Rok vydání:	2019
Předmět:	Blue dye biology Chemistry General Chemical Engineering Aspergillus niger Fractional factorial design biology.organism_classification Steepest ascent Glucose oxidase activity chemistry.chemical_compound Physisorption Surface response methodology Hydrogen peroxide Nuclear chemistry
Zdroj:	Revista Mexicana de Ingeniería Química. 18:701-714
ISSN:	2395-8472 1665-2738
Popis:	The dumping of dyes into bodies of water represents an environmental and health problem due to its toxicity. A solution to this problem could be the application of mycorremediation. So, in this work Aspergillus niger CDBB-H-175l was investigated in the discoloration (DC) of vat blue dye. A combination of statistical methodologies was applied to optimize DC; initiating with a 24-1 fractional factorial design (FFD), to evaluate; dye concentration, exposure time, pH and agitation. Results showed a minimum and maximum DC of 15% and 43%, respectively. By using a first-order model DC increased further to 63%. Additional experiments enhanced DC to 90% in step 7 of a steepest ascent design, and this was improved to 94% at a higher concentration of 521 mg/L with the application of surface response methodology. Of the 63% total DC determined in the first-order model, 28% was attributed to the glucose oxidase activity (16.5 U/mL) and the H2O2 produced by A. niger, and 72% due to physisorption to the cell wall. This is the first report where H2O2 produced by Aspergillus niger is implicated in dye decoloration.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::fd7f8c08e02624787e6390c279edfda6 https://doi.org/10.24275/uam/izt/dcbi/revmexingquim/2019v18n2/lira Zobrazit plný text záznamu