STEREOSELECTIVE HPLC DETERMINATION OF THYROXINE ENANTIOMERS IN PHARMACEUTICALS

Autor:	Maria Sucha, Daniel W. Armstrong, Ján Petrovaj, Tat’ána Gondová
Rok vydání:	2011
Předmět:	Detection limit Chromatography Calibration curve Chemistry Clinical Biochemistry Pharmaceutical Science Pharmaceutical formulation Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Stereoselectivity Methanol Enantiomer Triethylammonium acetate
Zdroj:	Journal of Liquid Chromatography & Related Technologies. 34:2304-2314
ISSN:	1520-572X 1082-6076
DOI:	10.1080/10826076.2011.587225
Popis:	Direct chiral liquid chromatographic method for the separation and determination of the thyroxine enantiomers in pharmaceutical preparations has been developed. Enantioselective separation was performed on the teicoplanin-based Chirobiotic T (250 mm × 4.6 mm, 5 µm) column in reversed-phase mode. The effects of the mobile phase composition, the pH, the temperature, and the flow rate on the chromatographic separation were investigated. Baseline separation (RS > 3.0) of the thyroxine enantiomers was obtained with methanol and 0.1% triethylammonium acetate, pH 4.0 (70:30, v/v) as isocratic mobile phase at 1.0 mL/min flow rate. UV detection was performed at 215 nm. The calibration curves of both thyroxine enantiomers were linear over the concentration range of 50–300 µg/mL. The limits of detection were 0.15 µg/mL and 0.20 µg/mL for L- and D-thyroxine, respectively. The method was validated in accordance with International Conference on Harmonization (ICH) guidelines. The proposed method was applied for the det...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::fd71445b04e7f70cac5be4f2634646f5 https://doi.org/10.1080/10826076.2011.587225 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.