Isolation of Ascomycota fungi with capability to transform PAHs: Insights into the biodegradation mechanisms of Penicillium oxalicum
| Autor: | Patricia Godoy, Mònica Rosell, Ernest Marco-Urrea, Elisabet Aranda, Rocío Reina, Inmaculada García-Romera, Marina Badia-Fabregat |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Anthracene Ascomycota Polycyclic aromatic hydrocarbon 010501 environmental sciences Biology Biodegradation Phenanthrene biology.organism_classification 01 natural sciences Microbiology Mineralization (biology) Biomaterials Dibenzofuran 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Dibenzothiophene Environmental chemistry Botany Waste Management and Disposal 0105 earth and related environmental sciences |
| Zdroj: | International Biodeterioration & Biodegradation. 122:141-150 |
| ISSN: | 0964-8305 |
| Popis: | The aim of this study was to gain insight into the ability of fungi present in a polycyclic aromatic hydrocarbon (PAH)-contaminated pond as remediation agents. Eight of the 10 strains isolated belonging to Ascomycota fungi and were able to remove anthracene after 21 days. Among them, Penicillium oxalicum was selected for further studies. Time course experiments showed that P. oxalicum completely removed anthracene and dibenzothiophene within 4 days, as well as phenanthrene and dibenzofuran, although at slower rates. Induction of oxygenase activity in the subcellular fraction of the fungal mycelium exposed to these aromatic compounds was deeply studied. Besides, the reduction of the conversion rate in the presence of a cytochrome P450 (CYP) inhibitor indicates a role in the first step of transformation of PAHs. The use of a defined growth medium with low carbon content for stable isotope tracer experiments using 13 C 6 -anthracene showed that glucose was required to proceed with anthracene degradation by P. oxalicum . Mineralization of anthracene could not be proved in these experiments, but 13 C-labelled oxy- and hydroxy-derivatives were identified by nuclear magnetic resonance (NMR) as major metabolites. These results show the potential application of the isolated fungus P. oxalicum in biotechnological pollutant removal processes. |
| Databáze: | OpenAIRE |
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