Study of Factors Affecting Molecular Behaviors in Phenothiazine-Mediated Biosensing by Electrochemical and Spectroscopic Methods

Autor:	Mindy Nguyen, John B. Sapp, Waldemar Gorski, Xin Wei, Yetunde Sorunmu, Tuan D. Phan
Rok vydání:	2006
Předmět:	biology Electrochemistry Combinatorial chemistry Amperometry Analytical Chemistry Chitosan chemistry.chemical_compound chemistry Covalent bond Phenothiazine biology.protein Organic chemistry Molecule Glucose oxidase Biosensor
Zdroj:	Electroanalysis. 18:2375-2380
ISSN:	1521-4109 1040-0397
DOI:	10.1002/elan.200603673
Popis:	Reagentless glucose-detecting biosensors were constructed by incorporating a series of phenothiazine derivatives as mediators onto chitosan (CHIT) matrix via different covalent bonds, wherein glucose oxidase (GOx) was employed as the enzyme. Electrochemical studies show significant decrease in the electrocatalytic current during cyclic voltammetric and amperometric measurements, resulting from complexes formation between GOx and phenothiazine molecules. This behavior was further verified by spectroscopic studies. The decrease in the peak intensity at 258 nm is due to the gradual complexes formation over time, consistent to the decrease in the current signal in electrochemical investigations. Correlation with the molecular structures of phenothiazine derivatives reveals a strong relationship between the hydrophobicity of the mediators and the stability of the biosensor electrodes.
Databáze:	OpenAIRE
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