Novel selective medium for the isolation ofcorynebacterium kroppenstedtiifrom heavily colonised clinical specimens
| Autor: | Vincent C.C. Cheng, Tiffany H Y Leung, Sally C. Y. Wong, Rosana W.S. Poon, CP Wong, Chuen-Hing Foo, Vivian C M Lam, Jonathan H. K. Chen, Kwok-Yung Yuen, Antonio H. Y. Ngan, Herman Tse |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
food.ingredient biology 030106 microbiology Corynebacterium General Medicine Granulomatous mastitis Fosfomycin medicine.disease biology.organism_classification Isolation (microbiology) Pathology and Forensic Medicine Mastitis Microbiology 03 medical and health sciences 0302 clinical medicine food 030220 oncology & carcinogenesis medicine Agar Bacteria medicine.drug Corynebacterium kroppenstedtii |
| Zdroj: | Journal of Clinical Pathology. 71:781-786 |
| ISSN: | 1472-4146 0021-9746 |
| DOI: | 10.1136/jclinpath-2017-204834 |
| Popis: | AimsGranulomatous mastitis due toCorynebacterium kroppenstedtiiis an increasingly recognised cause of an indolent and distressing mastitis in non-lactating females. This slow-growing lipophilic organism is not reliably isolated using routine culture methods. A novel selective culture medium (CKSM) is designed to optimise the isolation of this organism from clinical specimens.MethodsCKSM contains 10% galactose and Tween 80 (10%) to enhance the growth ofC. kroppenstedtii, fosfomycin (100 µg/mL) to suppress the other bacteria, and differentiateC. kroppenstedtiifrom non-kroppenstedtiilipophilic corynebacteria by esculin hydrolysis. The medium was evaluated for its ability to support the growth ofC. kroppenstedtii, selection and differentiation ofC. kroppenstedtiifrom other bacteria in non-sterile clinical specimens.ResultsC. kroppenstedtiigrew as 1–2 mm colonies with black halo on CKSM within 72 hours of incubation, compared with barely visible pinpoint colonies on routine blood agars. During the four-month period of evaluation with 8896 respiratory specimens, 103 breast specimens, 1903 female genital tract specimens, 617 newborn surface swabs and 10 011 miscellaneous specimens, 186C. kroppenstedtiiwere isolated, including 127 (1.4%) respiratory and 59 (0.5%) miscellaneous specimens, 184 of them were found only on CKSM. Besides the three (2.9%) positive breast specimens, 27 (1.4%) high vaginal and endocervical swabs, and 11 (1.8%) surface swabs of newborns were positive forC. kroppenstedtii.ConclusionsCKSM is a useful addition to routine agar media for the isolation ofC. kroppenstedtii,and will be helpful for studying the epidemiology and transmission of this unusualCorynebacteriumcausing granulomatous mastitis. |
| Databáze: | OpenAIRE |
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