Autor: |
B. Girase, Mahesh C. Somani, Leo Pentti Karjalainen, Thomas C. Pesacreta, Raja Devesh Kumar Misra, P.K.C. Venkatsurya |
Rok vydání: |
2012 |
Předmět: |
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Zdroj: |
Materials Science and Engineering: C. 32:330-340 |
ISSN: |
0928-4931 |
DOI: |
10.1016/j.msec.2011.10.036 |
Popis: |
From the perspective of osseointegration, nanograined/ultrafine-grained (NG/UFG) metals provide surfaces that are different from conventional coarse-grained (CG) polycrystalline metals because of the high fraction of grain boundaries. We describe here the interplay between the cellular response and grain boundary grooving as a potential approach to enhance osteoblast functions and facilitate the biomechanical interlocking and anchorage. This is accomplished by making a relative comparison of osteoblast response of NG/UFG grains electrochemically grooved to different depths to induce different degree of nanoscale roughness with planar NG/UFG surfaces, under identical biological environment. Electrochemically grooved NG/UFG structures indicated significant attachment and proliferation, and consequently enhanced modulation of cellular response that was significantly different from planar (non-grooved) NG/UFG substrate. Consistent with cell attachment and proliferation, immunofluorescence microscopy and computational analysis indicated stronger vinculin signals associated with actin stress fibers in the outer regions of the cells and cellular extensions on electrochemically-grooved NG/UFG substrates. These observations are indicative of accelerated response of cell–substrate interaction and activity. The behavior is attributed to average nanoscale roughness and high surface hydrophilicity of the nanoengineered surface. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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