DNA Fingerprinting of Some Mango (Mangifera indica L) Cultivars Using Anchored-ISSR Markers

Autor:	Ambika B. Gaikwad, J. L. Karihaloo, Sunaina Singh
Rok vydání:	2007
Předmět:	Jaccard index Dendrogram UPGMA Plant Science Biology Horticulture Similarity (network science) DNA profiling Genetic marker Botany Mangifera Cultivar Agronomy and Crop Science Biotechnology
Zdroj:	Journal of Plant Biochemistry and Biotechnology. 16:113-117
ISSN:	0974-1275 0971-7811
DOI:	10.1007/bf03321984
Popis:	Twelve Indian mango cultivars were fingerprinted using anchored-ISSR primers. Out of total 161 bands amplified, 113 (70.2%) were polymorphic, the polymorphism ranging from 50% to 94.1% depending upon the primer. One primer [5′ HVH(CA)7T 3′] with highest genotype index could uniquely identify each of the cultivars studied. Fingerprints based on polymorphic markers amplified by the 10 primers had a 6.75 x 10-12 probability of identical match by chance, indicating a high degree of uniqueness in the anchored-ISSR based fingerprinting. UPGMA dendrogram based on Jaccard’s similarity showed Himayath as the most diverse of the 12 cultivars and the rest of the cultivars clustered into two groups at 0.69 similarity coefficient.
Databáze:	OpenAIRE
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