Dramatic increase in readthrough acetylcholinesterase in a cellular model of oxidative stress
| Autor: | Ricarda Härtl, Martina Zimmermann, Anne S. Gleinich |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
Aché medicine.disease_cause Biochemistry Acetylcholinesterase language.human_language Cellular and Molecular Neuroscience chemistry.chemical_compound Endocrinology chemistry Apoptosis Cell culture Internal medicine Lactate dehydrogenase medicine language Viability assay Intracellular Oxidative stress |
| Zdroj: | Journal of Neurochemistry. 116:1088-1096 |
| ISSN: | 0022-3042 |
| DOI: | 10.1111/j.1471-4159.2010.07164.x |
| Popis: | J. Neurochem. (2011) 116, 1088–1096. Abstract Moderate, transient oxidative stress is achieved in SH-SY5Y cells using tertiary butylhydroperoxide as oxidant. Over a recovery period of 24 h, the enzymatic activity and protein levels of the acetylcholinesterase (AChE) splice variants tailed AChE (AChE-T) and readthrough AChE (AChE-R) are monitored. Their time-dependent correlation to pro- and anti-apoptotic factors, namely caspase 3 and Bcl-2, respectively, as well as lactate dehydrogenase release as a measure of cell viability is assessed. A distinctly different expression pattern of AChE-T as compared with AChE-R is recorded, in that AChE-T shows only a very slight increase over a 6 h time period. In contrast, AChE-R rises continuously during the recovery period, reaching peak intracellular levels that are up to six times higher than control levels 3–4 h post-stress, and is released from cells in substantial amounts. Moreover, anti-apoptotic Bcl-2 increases significantly, peaking 2–3 h after this AChE-R peak has occurred. We believe this study presents the first work that demonstrates – without relying on techniques of over-expression – the time-dependent correlation between apoptotic processes and related rescue mechanisms involving AChE isoforms in a neuronal cell line. |
| Databáze: | OpenAIRE |
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