Use of Specific Properties of Poly-N-vinylcaprolactam in Immunochemical Reactions

Autor:	T. B. Shumova, I. P. Chikhacheva, O. F. Khokhlachev, O. B. Novikova
Rok vydání:	2018
Předmět:	chemistry.chemical_classification Chromatography 010405 organic chemistry General Chemical Engineering HYDROSOL General Chemistry Polymer 010402 general chemistry 01 natural sciences Smart polymer 0104 chemical sciences Agglutination (biology) chemistry Antigen Poly-N-vinylcaprolactam
Zdroj:	Russian Journal of Applied Chemistry. 91:325-329
ISSN:	1608-3296 1070-4272
Popis:	Principal possibility of using a smart polymer, poly-N-vinylcaprolactam, for immobilization of biopharmaceuticals (antibodies and antigens) in the course of phase transition of a soluble coil into an insoluble globule was demonstrated. A procedure for preparing a hydrosol stable at room temperature in the presence of a stabilizer was examined. The hydrosol obtained, in which the collapsed polymer with the captured antigen acts as a solid support, can be used for agglutination with a complementary antibody, i.e., the immunochemical reaction can be evaluated visually.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::fd27250251ce0f84454f9f6fc7de79c8 https://doi.org/10.1134/s1070427218020246 Zobrazit plný text záznamu Full text from SpringerLink