Simple Spectrophotometric Sequential Injection Analysis System for Determination of Serum Calcium

Autor:	Chakorn Chinvongamorn, Wanida Laiwattanapaisal, Orawon Chailapakul, Yuwadee Boonyasit
Rok vydání:	2012
Předmět:	Detection limit Chromatography medicine.diagnostic_test Chemistry Magnesium Bilirubin chemistry.chemical_element Arsenazo III Calcium Absorbance Psychiatry and Mental health chemistry.chemical_compound Linear range Spectrophotometry medicine
Zdroj:	American Journal of Analytical Chemistry. :131-137
ISSN:	2156-8278 2156-8251
Popis:	A sequential injection analysis (SIA) system for the determination of total calcium in blood has been implemented. The assay principle is based on the arsenazo III method, in which the indicator’s complex with calcium forms a blue-purple colour. The absorbance peak height at 650 nm was proportional to the calcium content in samples. A linear range was obtained from 2.5 - 15 mg?dL–1 (r2 = 0.999), with a detection limit of 0.430 mg?dL–1 (3σ). Magnesium, glucose, hae-moglobin, and bilirubin at 123, 1,000, 50, and 0.5 mg?dL–1, respectively, were not found to interfere with the proposed system significantly (recovery 99.1% - 104.4%). The sampling frequency was 30 h–1, in which the carry-over effect was negligible (0.69%). The proposed SIA system was successfully applied to the determination of calcium from serum samples (r2 = 0.970; n = 30).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::fd21bda0fea2b695a937fbaca12b348f https://doi.org/10.4236/ajac.2012.32019 Zobrazit plný text záznamu