Dodecylamine Derivative of Hydroxocobalamin Acts as a Potent Inhibitor of Cobalamin-Dependent Methionine Synthase in Mammalian Cultured COS-7 Cells

Autor:	Tsuyoshi Ichiyanagi, Ryoichi Yamaji, Tomohiro Bito, Yoshihisa Nakano, Toshio Iwaki, Fumio Watanabe, Hiroshi Inui, Yukinori Yabuta, Mariko Yasui
Rok vydání:	2014
Předmět:	chemistry.chemical_classification biology Cell Hydroxocobalamin Molecular biology Enzyme assay Enzyme Mutase medicine.anatomical_structure Biochemistry chemistry Enzyme inhibitor biology.protein medicine Methionine synthase Cytotoxicity medicine.drug
Zdroj:	Food and Nutrition Sciences. :1318-1325
ISSN:	2157-9458 2157-944X
DOI:	10.4236/fns.2014.514143
Popis:	We evaluated whether the dodecylamine derivative of hydroxocobalamin acts as a potent inhibitor of cobalamin-dependent enzymes in an African green monkey kidney cell, COS-7. When the dodecylamine derivative (1.0 μmol/L) did not show any cytotoxicity in the cultured cells, the derivative could not affect methylmalonyl-CoA mutase (holo-enzyme) activity, but significantly inhibit methionine synthase (holo-enzyme) activity in the cell homogenates of COS-7 grown in 1.0 μmol/L hydroxocobalamin-supplemented medium. An immunoblot analysis indicated that the dodecylamine derivative could not decrease the protein level of methionine synthase, but significantly inhibit the enzyme activity.
Databáze:	OpenAIRE
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