The Dimethoxytrityl Resin Product Anchored Sequential Synthesis Method (DMT PASS): A Conceptually Novel Approach to Oligonucleotide Synthesis

Autor:	and David P. Sebesta, Randall S. Smith, Wolfgang Pieken, Gretchen M. Pratt, Kurt Vagle, Christopher J. Tompkins, T. Brian Hurley, John Yegge, Jeffery C. Mihaichuk
Rok vydání:	2000
Předmět:	chemistry.chemical_classification chemistry.chemical_compound Monomer chemistry Process development Oligonucleotide Product (mathematics) Organic Chemistry Organic chemistry Nucleotide Physical and Theoretical Chemistry Oligonucleotide synthesis Functionalized polystyrene
Zdroj:	Organic Process Research & Development. 4:214-224
ISSN:	1520-586X 1083-6160
DOI:	10.1021/op990095t
Popis:	Presented is a conceptually novel approach to oligonucleotide synthesis referred to herein as dimethoxytrityl resin product anchored sequential synthesis (DMT PASS). The DMT PASS process is characterized by the reaction of a 3‘-protected nucleoside or oligonucleotide with an excess of a nucleotide 3‘-phosphoramite or H-phosphonate which is bound to a dimethoxytrityl functionalized polystyrene resin. As a result, successfully coupled oligonucleotide product is then attached to the solid support, allowing for removal and potential recovery of starting materials. The protected oligonucleotide product is then released and subjected to an aqueous/organic extractive purification, which serves to remove monomeric impurities. The PASS process is anticipated to provide for the cost-effective manufacture of oligonucleotides on a scale which would allow for clinical development and pharmaceutical product commercialization. Herein we describe some aspects of our process development progress and discuss preliminary ap...
Databáze:	OpenAIRE
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