AN ISOTOPE DILUTION HIGH-RESOLUTION MASS SPECTROMETRY METHOD FOR QUANTITATIVE MEASUREMENT OF ISOMERIC BENZO[A]PYRENE TETROL METABOLITES DERIVED FROM ALBUMIN-BAPDE ADDUCTS AS INDICATORS OF HUMAN EXPOSURE TO POLYCYCLIC AROMATIC HYDROCARBONS

Autor: Donald G. Patterson, Kenroy E. Crawford, Alisha A. Etheredge, James Grainger, Christopher W. Davies, Miranda Hallett, Angela D. Ragin
Rok vydání: 2008
Předmět:
Zdroj: Polycyclic Aromatic Compounds. 28:434-450
ISSN: 1563-5333
1040-6638
DOI: 10.1080/10406630802378243
Popis: We evaluated in a pilot study a newly developed method of gas chromatography isotope dilution high-resolution mass spectrometry selected ion monitoring (GC-ID-HRMS-SIM). This method measures benzo[a]pyrene (B[a]P) tetrol metabolites released after albumin-BaPDE adduct hydrolysis. We isolated albumin adducts from the blood of a cohort of adult male and female smokers and nonsmokers randomly selected as exposed and nonexposed groups. Isomeric B[a]P tetrols released after adduct hydrolysis and silyl derivatization were quantified by GC-ID-HRMS-SIM using 13 C 6 -isotopically labeled BaP tetrol isomer standards (+/−)-BaP-r-7,t-8,t-9,c-10-tetrol (BPTI-1), (+/−)-BaP-r-7,t-8,t-9,t-10-tetrol (BPTI-2), (+/−)-BaP-r-7,t-8,c-9,t-10-tetrol (BPTII-1) and (+/−)-BaP-r-7,t-8,c-9,c-10-tetrol (BPTII-2). In all donor samples analyzed the method was sensitive enough to detect BPTII-1 and BPTI-1 in the low fmol range. In both smokers and nonsmokers BPTI-1 levels were higher than BPTII-1 levels. The mean levels of BPTII-1 and BP...
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje