Competitive enzyme-linked immunoassay for Factor VIII antigen

Autor:	L D Yorde, D E Yorde, Edward A. Sasse, C V Hussey
Rok vydání:	1979
Předmět:	Antiserum Chromatography biology medicine.diagnostic_test Chemistry Biochemistry (medical) Clinical Biochemistry Molecular biology Horseradish peroxidase Immune complex Microtiter plate Antigen hemic and lymphatic diseases Immunoassay biology.protein medicine Antibody Peroxidase
Zdroj:	Clinical Chemistry. 25:1924-1927
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/25.11.1924
Popis:	We describe a competitive enzyme-linked immunoassay for Factor VIII antigen. Binding of anti-factor VIII to solid-phase Factor VIII antigen is competitively inhibited by the free factor VIII antigen that is to be measured. The amount of anti-Factor VIII bound to solid-phase VIII is measured by applying in sequence a heterologous bridging antibody and a soluble antibody/enzyme immune complex. The soluble complex used was rabbit antiperoxidase/horseradish peroxidase. Peroxidase activity is inversely proportional to the Factor VIII antigen concentration in the original test plasma and is measured spectrophotometrically. The assay can be performed in as little as 4 h with only a microtiter plate, antisera, antigen, and a spectrophotometer. It is sensitive to 0.05 units of Factor VIII antigen per milliliter, and reproducibility, linearity, and normal range are similar to those reported for other techniques.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::fcf0843f16322d7cb8fa94130891beb4 https://doi.org/10.1093/clinchem/25.11.1924 Zobrazit plný text záznamu