Polymorphism of BoLA DRB3.2 Gene in Indian Holstein Crossbred Cows using PCR-RFLP
| Autor: | Satyender Singh Tomar, Kauvery Tayeng, Sunil Kumar Nagoriya, Vinita Mangrole, Sherly Ignetious |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
021110 strategic
defence & security studies education.field_of_study 05 social sciences Population 0211 other engineering and technologies Locus (genetics) 02 engineering and technology Biology Major histocompatibility complex Molecular biology Restriction enzyme Antigen Genotype 050501 criminology biology.protein Restriction fragment length polymorphism education Gene 0505 law |
| Zdroj: | International Journal of Livestock Research. :1 |
| ISSN: | 2277-1964 |
| DOI: | 10.5455/ijlr.20180826072302 |
| Popis: | Bovine lymphocyte antigen DRB3.2 (BoLA–DRB3.2) gene encodes for the beta chain of the major histocompatibility complex (MHC) class II molecule in cattle, which is a glycoprotein present on the surface of antigen-presenting cells. The objective of the present study was to genotype the BoLA-DRB3.2 locus in Holstein Crossbred cattle (n = 40) by PCR-RFLP. The 304bp amplified region was separately digested with restriction endonucleases Hae III and Bst YI. Seven BoLA-DRB 3.2 genotypes were detected by digesting with Hae III. The frequencies of the genotypes were: 0.475, 0.225, 0.050, 0.100, 0.050, 0.050 and 0.050 for the genotype AA, AB, AE, BB, DD, EE and II, respectively. Digestion with Bst YI yielded three genotypes AA, AB and BB with frequency of 0.050, 0.625 and 0.325. On the basis of the above results it can be concluded that the DRB3.2 gene was found to be highly polymorphic in the crossbred cattle population |
| Databáze: | OpenAIRE |
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