Therapeutic effects of Hedyotis�diffusa Willd in a COPD mouse model challenged with LPS and smoke
| Autor: | Qi Wang, Huan He, Juan Chen, Peihong Wang, Yongtao Xie, Jianming Liu, Chengxin Li, Jing Zhu, Renping Liu, Caiqing Wu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research Lipopolysaccharide Pharmacology Hedyotis diffusa 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Immunology and Microbiology (miscellaneous) Medicine Dexamethasone medicine.diagnostic_test biology business.industry Interleukin General Medicine biology.organism_classification 030104 developmental biology Bronchoalveolar lavage chemistry Apoptosis 030220 oncology & carcinogenesis Tumor necrosis factor alpha business Transforming growth factor medicine.drug |
| Zdroj: | Experimental and Therapeutic Medicine. |
| ISSN: | 1792-1015 1792-0981 |
| Popis: | Hedyotis diffusa Willd (HDW) is a constituent of several Chinese medicines used clinically to treat inflammatory diseases, including airway inflammation. The aim of the present study was to investigate whether HDW serves a protective role in suppressing chronic airway inflammation and its underlying mechanisms. A mouse model of chronic smoking was induced via exposure to cigarette smoke (CS) for 30 days, increasing the exposure time for up to 5 min per day and the administration of lipopolysaccharide (LPS). Mice were gavaged with HDW (50 or 100 mg/kg body weight), dexamethasone (1 mg/kg body weight) or normal saline (NS, 0.9%) 1 h prior to CS challenge. Compared with CS and LPS (SL)-induced mice, the levels of interleukin (IL)-1β, tumor necrosis factor-α and transforming growth factor-β in bronchoalveolar lavage fluid from HDW+SL mice were significantly decreased and IL-10 was markedly reduced. Histological examination of the lung tissues revealed that HDW treatment alleviates airway inflammation. In addition, the administration of HDW to human bronchial epithelial BEAS-2B cells suppressed the activity of the nuclear factor (NF)-κB signaling pathway. The results of the present study demonstrate that HDW has a therapeutic effect in COPD and the underlying mechanism may be attributed to inhibition of the NF-κB pathway. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|