Integration of mandarin (Citrus reticulata) cytogenetic map with its genome sequence
| Autor: | Javier Terol, Walter dos Santos Soares Filho, Manuel Talon, Thallita Régis, Andrea Pedrosa-Harand, Sandra Mendes |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Whole genome sequencing Genetics Bacterial artificial chromosome Heterochromatin food and beverages General Medicine Biology 01 natural sciences Genome Cytogenetic map Mandarin Chinese language.human_language 03 medical and health sciences 030104 developmental biology Genus language Molecular Biology 010606 plant biology & botany Biotechnology |
| Zdroj: | Genome. 63:437-444 |
| ISSN: | 1480-3321 0831-2796 |
| DOI: | 10.1139/gen-2020-0046 |
| Popis: | Citrus is an extremely important genus in terms of world fruit production. Despite its economic importance and the small genome sizes of its species (2n = 18, 1C = 430 ± 68 Mbp), entire genomic assemblies have only recently become available for some of its representatives. Together with the previous CMA/DAPI banding and fluorescence in situ hybridization (FISH) in the group, these data are important for understanding the complex relationships between its species and for assisting breeding programs. To anchor genomic data with the cytogenetic map of mandarin (Citrus reticulata), the parental species of several economically important hybrids such as sweet orange and clementine, 18 BAC (bacterial artificial chromosome) clones were used. Eleven clementine BACs were positioned by BAC-FISH, doubling the number of chromosome markers so far available for BAC-FISH in citrus. Additionally, six previously mapped BACs were end-sequenced, allowing, together with one BAC previously sequenced, their assignment to scaffolds and the subsequent integration of chromosomes and the genome assembly. This study therefore established correlations between mandarin scaffolds and chromosomes, allowing further structural genomic and comparative study with the sweet orange genome, as well as insights into the chromosomal evolution of the group. |
| Databáze: | OpenAIRE |
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