Surface-modified functionalized polycaprolactone scaffolds for bone repair:In vitroandin vivoexperiments
| Autor: | Jonas Jensen, Helle Lysdahl, Moustapha Kassem, Jan Duedal Rölfing, Lea Bjerre Hokland, Jens Vinge Nygaard, Asger Albæk Kristiansen, Cody Bünger, Michael Bendtsen, Dang Quang Svend Le |
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| Rok vydání: | 2013 |
| Předmět: |
Scaffold
Foreign-body giant cell Materials science technology industry and agriculture Metals and Alloys Biomedical Engineering Calvaria macromolecular substances Bone healing equipment and supplies musculoskeletal system Bone morphogenetic protein 2 Osseointegration Resorption Biomaterials chemistry.chemical_compound medicine.anatomical_structure chemistry Polycaprolactone Ceramics and Composites medicine Biomedical engineering |
| Zdroj: | Journal of Biomedical Materials Research Part A. 102:2993-3003 |
| ISSN: | 1549-3296 |
| Popis: | A porcine calvaria defect study was carried out to investigate the bone repair potential of three-dimensional (3D)-printed poly-e-caprolactone (PCL) scaffolds embedded with nanoporous PCL. A microscopic grid network was created by rapid prototyping making a 3D-fused deposition model (FDM-PCL). Afterward, the FDM-PCL scaffolds were infused with a mixture of PCL, water, and 1,4-dioxane and underwent a thermal-induced phase separation (TIPS) followed by lyophilization. The TIPS process lead to a nanoporous structure shielded by the printed microstructure (NSP-PCL). Sixteen Landrace pigs were divided into two groups with 8 and 12 weeks follow-up, respectively. A total of six nonpenetrating holes were drilled in the calvaria of each animal. The size of the cylindrical defects was h 10 mm and O 10 mm. The defects were distributed randomly using following groups: (a) NSP-PCL scaffold, (b) FDM-PCL scaffold, (c) autograft, (d) empty defect, (a1) NSP-PCL scaffold + autologous mononuclear cells, and (a2) NSP-PCL scaffold + bone morphogenetic protein 2. Bone volume to total volume was analyzed using microcomputed tomography (µCT) and histomorphometry. The µCT and histological data showed significantly less bone formation in the NSP-PCL scaffolds in all three variations after both 8 and 12 weeks compared to all other groups. The positive autograft control had significantly higher new bone formation compared to all other groups except the FDM-PCL when analyzed using histomorphometry. The NSP-PCL scaffolds were heavily infiltrated with foreign body giant cells suggesting an inflammatory response and perhaps active resorption of the scaffold material. The unmodified FDM-PCL scaffold showed good osteoconductivity and osseointegration after both 8 and 12 weeks. |
| Databáze: | OpenAIRE |
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