Platelet Antibody Analysis by Three Different Tests
| Autor: | Gerhard Lanzer, Susanne Macher, Ursula Posch, Camilla Drexler, Katharina Schallmoser, Nazanin Sareban |
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| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
chemistry.chemical_classification Weakly positive biology business.industry Biochemistry (medical) Clinical Biochemistry Public Health Environmental and Occupational Health Hematology Human leukocyte antigen Molecular biology Platelet antibody Medical Laboratory Technology chemistry Monoclonal Immunology biology.protein Retrospective analysis Immunology and Allergy Medicine Platelet Antibody Glycoprotein business |
| Zdroj: | Journal of Clinical Laboratory Analysis. 29:198-202 |
| ISSN: | 0887-8013 |
| DOI: | 10.1002/jcla.21750 |
| Popis: | Background Platelet-reactive antibodies lead to thrombocytopenia and bleeding disorders, and diverse assays are used for their detection. In this retrospective analysis, the applicability of three different test systems was compared and antibody specificities were assessed. Methods Sera of 1,234 patients were tested with an enzyme-linked immunosorbent assay (ELISA; Lifecodes PAKPLUS® or PAK 12®, Gen-Probe) and a solid-phase assay (Capture-P Ready Screen®, Immucor Inc.). In cases of suspected anti-HLA class I antibodies, a specific lymphocytotoxicity test (LCT, Bio-Rad®) was performed. Results Platelet antibodies were detected in 366 of 1,234 samples (29.7%). In 70.3% concordant negative but only in 8.4% concordant positive results were obtained with both the methods; 185 of 1,053 in the solid-phase assay negative samples were positive in the ELISA (15.0%). In samples positive in both methods, most antibodies reacted against HLA class I antigens. Glycoprotein (GP) specific platelet antibodies, mainly against GPIIb/IIIa and GPIa/IIa, were more frequently detectable in the ELISA than in the solid-phase assay, whereas weakly positive results have to be interpreted cautiously. Conclusion ELISA, solid-phase assay, and LCT showed highly divergent results. Due to several limitations, the additional analysis by the “monoclonal antibody-specific immobilization of platelet antigen” (MAIPA)—assay is highly recommended. |
| Databáze: | OpenAIRE |
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