Optimization of culture conditions for the production of polysaccharides and kinsenoside from the rhizome cultures of Anoectochilus roxburghii (Wall.) Lindl

Autor: Li Qiu Zhang, Mei Lan Lian, Ri Gao, Mei Yu Jin, Xuan Chun Piao
Rok vydání: 2018
Předmět:
Zdroj: In Vitro Cellular & Developmental Biology - Plant. 54:25-35
ISSN: 1475-2689
1054-5476
Popis: The present study designed two sets of experiments by using the uniform design method and investigated the effects of medium components on the accumulation of bioactive compounds (polysaccharide and kinsenoside) in rhizomes, in order to select a suitable culture medium for the rhizome suspension culture of Anoectochilus roxburghii (Wall.) Lindl. Among the combinations of Murashige and Skoog (MS) medium strengths and plant growth regulator (benzylaminopurine, BA; kinetin, KT; and α-naphthaleneacetic acid, NAA) concentrations, and the combinations of nitrogen, phosphorus, and sucrose concentrations, the maximum yield of polysaccharides and kinsenoside was achieved with 0.75 × MS + 2.0 mg L−1 BA + 0.2 mg L−1 KT + 0.5 mg L−1 NAA and 45 mM nitrogen + 0.93 mM phosphorus + 35 g L−1 sucrose, respectively. Therefore, the optimal rhizome suspension culture medium was 0.75 × MS medium supplemented with 2.0 mg L−1 BA, 0.2 mg L−1 KT, 0.5 mg L−1 NAA, and 35 g L−1 sucrose. Yeast extract (YE) enhanced bioactive compound accumulation in rhizomes. The polysaccharide and kinsenoside production was significantly improved when 75 mg L−1 YE was added to the culture medium after 30 d of rhizome suspension culture; 8.3 g L−1 of polysaccharide and 6.1 g L−1 of kinsenoside were obtained after 4 d of YE treatment. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of YE-treated rhizomes was higher than that of YE-untreated rhizomes, demonstrating enhanced antioxidant activity of the treated bioreactor-cultured rhizomes.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje