Development of primer sets for multiplex and qPCR assays targeting Skeletonema species and their application to field samples
| Autor: | Mayuko Otsubo, Tomomi Hamasaki, Yutaro Ando, Katsuhide Yokoyama, Machiko Yamada, Yuki Yoshinaka, Emika Miyamura, Natsumi Enjoji, Fuka Furuoka, Toshiya Katano |
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| Rok vydání: | 2019 |
| Předmět: |
010504 meteorology & atmospheric sciences
Brackish water biology 010505 oceanography Zoology Skeletonema potamos Oceanography biology.organism_classification 01 natural sciences Tidal zone Salinity Diatom Multiplex polymerase chain reaction Multiplex Primer (molecular biology) 0105 earth and related environmental sciences |
| Zdroj: | Journal of Oceanography. 75:319-334 |
| ISSN: | 1573-868X 0916-8370 |
| DOI: | 10.1007/s10872-018-00504-3 |
| Popis: | Skeletonema is a dominant diatom, especially in coastal waters. However, species identification and quantification in the natural environment has not yet been established, as species identification can be nearly impossible with light microscopy. In the present study, we developed primer sets for multiplex and real-time PCR to identify and enumerate the 10 Skeletonema species, and applied this technique to samples obtained from the tidal zone of the Chikugo River and its adjacent waters of the Ariake Sea from spring to summer. In total, eight Skeletonema species were detected during the investigation. Among the eight, the S. marinoi–dohrnii complex was detected at all three stations. Skeletonema potamos was the second most frequently detected, mainly in the Chikugo River. The overall number of detected species was low at the upper tidal zone of the Chikugo River, where salinity was |
| Databáze: | OpenAIRE |
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