Isolation of Mitochondria from Saccharomyces cerevisiae
| Autor: | Toshiaki Izawa, Ann Katrin Unger |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
biology ved/biology ved/biology.organism_classification_rank.species Saccharomyces cerevisiae food and beverages Oxidative phosphorylation Spheroplast Mitochondrion biology.organism_classification Yeast 03 medical and health sciences 030104 developmental biology Biochemistry biology.protein Translocase Model organism Function (biology) |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493968220 |
| DOI: | 10.1007/978-1-4939-6824-4_3 |
| Popis: | The budding yeast Saccharomyces cerevisiae is an important model organism to study cellular structure and function. Due to its excellent accessibility to genetics and biochemical and microscopic analyses, studies with yeast have provided fundamental insights into mitochondrial biology. Yeast offers additional advantages because it can grow under fermenting conditions when oxidative phosphorylation is not obligatory and because the majority of mitochondrial structure and function are largely conserved during evolution. Isolation of mitochondria is an important technique for mitochondrial studies. This chapter focuses on procedures for the isolation and purification of intact yeast mitochondria that can be used for numerous functional assays as well as for analyses of mitochondrial ultrastructure. |
| Databáze: | OpenAIRE |
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