Purine Nucleoside Phosphorylase-Catalyzed, Phosphate-Independent Hydrolysis of 2-Amino-6-mercapto-7-methylpurine Ribonucleoside
| Autor: | Eugene H. Cordes, Victor Farutin, Gayatry G. Jacob-Mosier, Zhijun Wu, Brad Riley, Jianming Cheng, Ryan Hakimi |
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| Rok vydání: | 1999 |
| Předmět: |
inorganic chemicals
Chemistry Stereochemistry Organic Chemistry Purine nucleoside phosphorylase Guanosine Trimer Ribonucleoside Phosphate Biochemistry Dissociation constant enzymes and coenzymes (carbohydrates) Hydrolysis chemistry.chemical_compound Drug Discovery medicine heterocyclic compounds Inosine Molecular Biology medicine.drug |
| Zdroj: | Bioorganic Chemistry. 27:307-325 |
| ISSN: | 0045-2068 |
| DOI: | 10.1006/bioo.1999.1137 |
| Popis: | In the presence of 1 mM phosphate, 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG) is a well-behaved substrate for calf spleen purine nucleoside phosphorylase (PNP). In the absence of phosphate, calf spleen PNP catalyzes a slow hydrolysis of MESG, which is accompanied by inactivation of the enzyme, analogous to the previously observed PNP-catalyzed hydrolysis of inosine and guanosine with formation, in the former case, of a stable PNP·hypoxan thine complex (P. C. Kline and V. L. Schramm (1992) Biochemistry 31 , 5964–5973). Qualitative and semiquantitative features of calf spleen PNP-catalyzed hydrolysis of MESG are accounted for by the following model. First, in the absence of phosphate and at pH 7.4, the enzyme exists as an equilibrium mixture of monomer and trimer with a dissociation constant for the trimer of 3 × 10 14 M 2 . Second, a stoichiometric reaction between three molecules of MESG and the PNP trimer results in the formation of a stable PNP·purinethiol complex. Third, the PNP·purinethiol complex initially formed with the monomeric enzyme partitions between product release and formation of a stable complex with 55 turnovers per inactivation event. Fourth, the stable PNP·purinethiol complexes are rapidly dissociated by phosphate to regenerate active enzyme. This dissociation is accompanied by an increase in absorbance at 356 nm consistent with a p K a for the purinethiol base on the enzyme of 8.1, compared to a corresponding value of 8.8 in aqueous solution. |
| Databáze: | OpenAIRE |
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