| Popis: |
Inhibition of melanogenesis by quercetin and vitamin E are extensively reported in the literature, independently, with the limitations of their antioxidant potential owing to less permeation, slight solubility in water, decreased bioavailability and reduced stability. Aim of the present study was to enhance antioxidant properties of quercetin by the incorporation of zinc and copper that was proved by docking study, along with investigating the synergistic effect of vitamin E loaded nanoformulation. Optimized metallic complexes of quercetin was additionally used in graftingnovel transdermal absorbable nanoparticles of polycaprolactone with Vit. E (PCL-NPs, Q-PCL-NPs, Zn-Q-PCL-NPs, Cu-Q-PCL-NPs). Physiochemical analysis was used to characterize nanoparticles, polyphenol-metal-Vitamin E conjugation was strengthened by FTIR, zetasizer and PDI. Percentage release of vitamin E was 80 ± 0.54% in case of Cu-Q-PCL-NPs-E which may be due to increase in particle size even after 8h. Non-cellular antioxidant effect by DPPH was observed 93 ± 0.23% in Cu-Q-PCL-NPs-E which was double as compared to Zn-Q-PCL-NPs-E. Reactive oxidative spices (ROS) for cellular antioxidant properties on MCF-7 cell was observed 90 ± 0.32% with the addition of its anticancer behavior 89 ± 0.64% of Cu-Q-PCL-NPs-E after 6 and 24h. Interestingly, 80 ± 0.53% inhibition of melanocytes cells and 95 ± 0.54% increases of keratinocytes cells that proved the tyrosinase enzyme inhibition of Cu-Q-PCL-NPs-E with their 17 months shelf life. Conclusively, use of pure quercetin and its copper complex in simple and in vitamin E loaded nanoparticles, can provide better antioxidant properties with inhibition of melanin, which can be utilized in treatment of melanogenesis disease. |
