Genome sequencing of the Monkeypox virus 2022 outbreak with Amplicon-based Oxford Nanopore MinION sequencing

Autor: Annika Brinkmann, Katharina Pape, Steven Uddin, Niklas Woelk, Sophie Förster, Heiko Jessen, Janine Michel, Claudia Kohl, Lars Schaade, Andreas Nitsche
Rok vydání: 2022
DOI: 10.1101/2022.10.20.512862
Popis: We present an amplicon-based assay for MinION Nanopore sequencing of monkeypox virus genomes from clinical specimens, obtaining high-quality results for up to 99 % of the genomes, even for samples with high Ct values of up to 30 which are not suitable for shotgun sequencing.
Databáze: OpenAIRE