IDENTIFICATION AND CHARACTERIZATION OF NEUTRAL PROTEASE PRODUCING Paenibacillus Polymyxa SPECIES EMBS024 BY 16S rRNA GENE SEQUENCING

Autor: Sheaza Ahmed, Anuraj Ns, Sneha Singh, Mukesh Yadav, Meer Asif A, Mayura Maynal, Urvashi Pisal
Rok vydání: 2012
Předmět:
Zdroj: International Journal of Microbiology Research. 4:236-239
ISSN: 0975-9174
0975-5276
Popis: The term probiotics means supporting life, just being on the opposite spectrum to antibiotic which is against life. Probiotic microor- ganisms are those microbially derived factors that stimulate the growth of other microorganisms. They are considered to be the best alterna- tive of antibiotics. The current study illustrate the identification of a neutral protease producing bacteria from soil by 16s rRNA sequencing technique. The sample was collected from outskirts of Vijayawada, Andhra Pradesh, India, over dump food wastes soil. The collected sam- ple was serially diluted and the targeted colony after incubation over a certain time period was introduced to 16s rRNA sequencing. The result showed the extract to be a neutral protease producing bacteria which was further named as Paenibacillus polymyxa strain EMBS024. After characterization the sequence of isolate was deposited in GenBank with accession number JQ004091.
Databáze: OpenAIRE