FUNCTIONAL STUDIES ON CELLS FROM HUMAN PEYER'S PATCHES. THEIR PHENOTYPE AND IN VITRO PROLIFERATIVE RESPONSES

Autor:	Jo Spencer, C B Williams, John Walker-Smith, Thomas T. MacDonald, Jo Viney
Rok vydání:	1986
Předmět:	Pathology medicine.medical_specialty medicine.diagnostic_test Cell Biology medicine.disease Inflammatory bowel disease Phenotype In vitro medicine.anatomical_structure Pediatrics Perinatology and Child Health Biopsy medicine Collagenase Cytotoxic T cell Pan-T antigens medicine.drug
Zdroj:	Pediatric Research. 20:689-689
ISSN:	1530-0447 0031-3998
Popis:	During routine diagnostic colonoscopy of children for suspected inflammatory bowel disease it is possible to see Peyer's patches in the terminal ileum. Biopsies were taken of the PP, the lymphocytes isolated by collagenase digestion, and compared with cells isolated from the colonic mucosa. A PP biopsy yielded 2-3 times as many lymphocytes as a biopsy of mucosa (0.6 × 106 from a mucosal biopsy and 1.5 × 106 from a PP biopsy). The frequency of cells bearing the markers UCHT1 (pan T cell), Leu3a (helper/inducer T cells), and UCHTA (suppressor/cytotoxic T cells) was the sane for the 2 cell populations. The major differences were that the PP cells contained B cells and the mucosal lymphocytes contained IgA plasma cells. When stimulated in vitro with PHA or PHM cells from both sites divided. This is the first report on the characteristics and function of human PP cells and will allow us now to determine if IgA specific T and B cells are found in the PP of man as has been reported for rodents.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::fc311bec0d6b6a1dedd864ce35544259 https://doi.org/10.1203/00006450-198607000-00024 Zobrazit plný text záznamu