Ca2+ uptake and plasma membrane depolarization associated with blue light-sensitive exogenous NADH oxidation by Cuscuta protoplasts
| Autor: | Prakash C. Misra, Neerja Masih |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
chemistry.chemical_classification
Cuscuta reflexa biology Physiology ATPase chemistry.chemical_element Depolarization Plant Science Calcium Hyperpolarization (biology) biology.organism_classification Enzyme chemistry Biochemistry Proton transport biology.protein Agronomy and Crop Science Ion transporter |
| Zdroj: | Journal of Plant Physiology. 158:29-34 |
| ISSN: | 0176-1617 |
| DOI: | 10.1078/0176-1617-00086 |
| Popis: | Summary Protoplasts isolated from the apical segments of Cuscuta reflexa exhibited blue light-sensitive PM-linked NADH oxidase activity and increased rate of Ca 2+ -uptake in presence of NADH in dark, which was also stimulated by blue light. Contrary to marginal inhibition by Con A treatment, the ATPase inhibitors significantly inhibited the Ca 2+ uptake by the protoplasts both in dark and under blue light. The Ca 2+ -calmodulin antagonists, W-7 and calmidazolium, also inhibited Ca 2+ -uptake by protoplasts under similar conditions. The state of PM polarization was monitored by the fluorescent dye 9-amino acridine. It was observed that PM-linked NADH oxidation caused hyperpolarization of the membrane, the exposure of which to blue light resulted in membrane depolarization. The presence of Ca 2+ -calmodulin antagonists or Con A treatment completely abolished the blue light-induced membrane depolarization. It is argued that these actities at the PM, having some glycoproteic components, are functionally closely involved in blue light-induced signal transduction in Cuscuta |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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