Effects of Antimicrobial Administration on the Prevalence of Antimicrobial-Resistant Escherichia coli in Broiler Flocks
| Autor: | Montira Yossapol, Michiyo Sugiyama, Tetsuo Asai, Kasumi Suzuki |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Colony-forming unit Veterinary medicine Sulfamethoxazole 030106 microbiology Broiler General Medicine Biology Antimicrobial biology.organism_classification Trimethoprim 03 medical and health sciences 0302 clinical medicine Infectious Diseases medicine 030212 general & internal medicine Flock Feces Bacteria medicine.drug |
| Zdroj: | Japanese Journal of Infectious Diseases. 72:179-184 |
| ISSN: | 1884-2836 1344-6304 |
| DOI: | 10.7883/yoken.jjid.2018.277 |
| Popis: | The increase in antimicrobial-resistant (AMR) bacteria caused by antimicrobial usage is a public health problem. We investigated the proportion of cephalexin (LEX)-resistant bacteria in fresh feces obtained from antimicrobial-free broilers in three flocks at ＜15, 15-40, and＞ 40 days old. DHL agar plates containing 25 μg/mL LEX (DHL-L) showed LEX-resistant bacteria in all flocks at ＜15 days old and in one flock at ＞ 40 days old. The bacterial counts on DHL and DHL-L were 105-108 colony forming units (CFU)/g feces and ＜102-105 CFU/g feces, respectively. We also assessed the proportion of AMR bacteria in feces collected at 5, 12, 19, 26, 33, and 40 days old from two flocks treated with amoxicillin at 5-7 days old and co-trimoxazole at 24-26 days old. The proportion of ampicillin (AMP)-resistant bacteria was elevated at 12 and 26-33 days old on DHL containing 50 μg/mL AMP, while no increase in LEX-resistant bacteria was observed on DHL-L. All isolates tested exhibited AMP resistance at 12 days old, while most exhibited resistance to both AMP and trimethoprim/sulfamethoxazole at 26-33 days old. Our results suggest that antimicrobial administration influenced the selection of AMR bacteria with cross- and coresistance. |
| Databáze: | OpenAIRE |
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