Surface Engineered Carboxymethylchitosan/Poly(amidoamine) Dendrimer Nanoparticles for Intracellular Targeting
| Autor: | João F. Mano, Hajime Ohgushi, Alexandra P. Marques, Motohiro Hirose, Joaquim M. Oliveira, Johan Benesch, Noriko Kotobuki, Rui L. Reis, Rogério P. Pirraco, Silgia A. Costa |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
0303 health sciences
Materials science Stromal cell Nanoparticle Nanotechnology 02 engineering and technology Poly(amidoamine) Cell sorting 021001 nanoscience & nanotechnology Condensed Matter Physics In vitro 3. Good health Electronic Optical and Magnetic Materials Biomaterials 03 medical and health sciences Tissue engineering Dendrimer Drug delivery Electrochemistry Biophysics 0210 nano-technology 030304 developmental biology |
| Zdroj: | Advanced Functional Materials. 18:1840-1853 |
| ISSN: | 1616-301X |
| DOI: | 10.1002/adfm.200800165 |
| Popis: | Novel highly branched biodegradable macromolecular systems have been developed by grafting carboxymethylchitosan (CMCht) onto low generation poly(amidoamine) (PAMAM) dendrimers. Such structures organize into sphere-like nanoparticles that are proposed to be used as carriers to deliver bioactive molecules aimed at controlling the behavior of stem cells, namely their proliferation and differentiation. The nanoparticles did not exhibit significant cytotoxicity in the range of concentrations below 1 mg mL -1 , and fluorescent probe labeled nanoparticles were found to be internalized with highly efficiency by both human osteoblast-like cells and rat bone marrow stromal cells, under fluorescence-activated cell sorting and fluorescence microscopy analyses. Dexamethasone (Dex) has been incorporated into CMCht/PAMAM dendrimer nanoparticles and release rates were determined by high performance liquid chromatography. Moreover, the biochemical data demonstrates that the Dex-loaded CMCht/PAMAM dendrimer nanoparticles promote the osteogenic differentiation of rat bone marrow stromal cells, in vitro. The nanoparticles exhibit interesting physicochemical and biological properties and have great potential to be used in fundamental cell biology studies as well as in a variety of biomedical applications, including tissue engineering and regenerative medicine. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text