| Popis: |
The inactivation rates of poliovirus (Lsc, 2ab strain) suspended in cell maintenance medium (MEM) and in phosphate buffer salt solution (PBS) with sodium hypochlorite (NaOCl), dichloro-, and trichloro-isocyanurates (2Cl-ICA, and 3Cl-ICA) and monochloramine (NH2Cl), 0.1-2.0 mg/l as available chlorine, were compared in the same conditions at pH 7 and 20°C. The formation of combined available chlorine (CA-Cl) with the virus suspension and the efficacy of the formed CA-Cl on the virus were studied. The amount of CA-Cl formed in the MEM suspension was greater than that in the PBS suspension and the virus was inactivated rapidly with free available chlorine (FA-Cl). In the PBS suspension containing 0.4 mg/l of chlorine, only a part of the chlorine convert to CA-Cl and more than 99% of the virus was inactivated in 3 min. In the MEM suspension containing the same concentration of chlorine, all of the chlorine was converted to CA-Cl and the inactivation of virus was not observed in 5 min. In 2.0 mg/l of chlorine with MEM suspension, about a half of FA-Cl remained and 99% of the virus was inactivated in 1 min. In 0.4 mg/l of NH2Cl, only 20% of the virus was inactivated in 5 min. The differences of CA-Cl formed in the virus suspension will give a significant variation in virus inactivation. There were no difference in CA-Cl formation in the virus suspension and the inactivation rate of the virus between hypochlorite and chloroisocyanurates. |
