Exopolysaccharide from Lactobacillus helveticus: identification of chemical structure and effect on biscuit duality
| Autor: | Manal G. Mahmoud, Mohsen S. Asker, Ahmed M.S. Hussein, Ghada Saber Ibrahim |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
food.ingredient
Lactobacillus helveticus Absorption of water biology 010405 organic chemistry Chemistry Food additive Food storage Wheat flour 02 engineering and technology 021001 nanoscience & nanotechnology biology.organism_classification 01 natural sciences 0104 chemical sciences food Food science 0210 nano-technology Sugar Food quality Ethanol precipitation Food Science |
| Zdroj: | Czech Journal of Food Sciences. 28:225-232 |
| ISSN: | 1805-9317 1212-1800 |
| DOI: | 10.17221/97/2009-cjfs |
| Popis: | Exopolysaccharide (LB-gum) was produced from Lactobacillus helveticus by ethanol precipitation and gel-permeation chromatography. The structures of LB1 and LB2 were estimated by sugar composition analysis, methylation, and FT-IR analysis. The results proved the contents of glucose and galactose in molar ratio of 2:1 and 2.3:1, and molecular weights ∼5.4 x 10 5 Da and ∼20.3 x 10 5 Da, respectively. Xanthan and LB-gum were added to wheat flour during biscuits making at levels of 0, 0.5, 1.0, 1.5, and 2.0%. Rheological properties and chemical quality attributes of the biscuits during storage for six months were evaluated. The data revealed slight increases in water absorption, dough development time, and dough stability but weakening decreased about 50 %. Also, height, weight, volume, and specific volume were found to increase as xanthan and LB-gum level increased. The colour was slightly affected. There were no significant differences between the samples containing xanthan and LB-gum at different levels with respect to all parameters tested except for the taste. Shelf - life of biscuit prolonged as a result of xanthan or LB-gum addition. The best addition level to improve the biscuits quality was 1.0% of xanthan or 1.5% of LB-gum. |
| Databáze: | OpenAIRE |
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