Perivenous expression of the mRNA of the three hypoxia-inducible factor α-subunits, HIF1α, HIF2α and HIF3α, in rat liver
| Autor: | Yvonne Cornesse, Thomas Kietzmann, Said Modaressi, K Brechtel, Kurt Jungermann |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
chemistry.chemical_classification
0303 health sciences Messenger RNA RNA Cell Biology Transfection Biology Biochemistry Molecular biology Amino acid Reverse transcription polymerase chain reaction 03 medical and health sciences 0302 clinical medicine Hypoxia-inducible factors chemistry 030220 oncology & carcinogenesis Rat liver Luciferase Molecular Biology 030304 developmental biology |
| Zdroj: | Biochemical Journal. 354:531-537 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj3540531 |
| Popis: | The cDNAs of three hypoxia-inducible factor (HIF) α-subunits were cloned from RNA of primary rat hepatocytes by reverse transcriptase PCR. All three cDNAs encoded functionally active proteins, of 825, 874 and 662 amino acids. After transfection they were able to activate luciferase activity of a luciferase gene construct containing three HIF-responsive elements. The mRNAs of the rat HIF α-subunits were expressed predominantly in the perivenous zone of rat liver tissue; the nuclear HIFα proteins, however, did not appear to be zonated. The sequence data reported here have been deposited in the EMBL Nucleotide Sequence Database under the following accession numbers: rHIF1α, Y09507; rHIF2α, AJ277828; rHIF3α, AJ277827. |
| Databáze: | OpenAIRE |
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