Relationship of neutrophil phagocytosis and oxidative burst with the subgingival microbiota of generalized aggressive periodontitis
| Autor: | P. X. Elsas, J. S. Mesquita, A. Bonomo, Ana Paula Vieira Colombo, R. P. M. Carvalho |
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| Rok vydání: | 2009 |
| Předmět: |
Microbiology (medical)
Periodontitis biology Phagocytosis Immunology Aggregatibacter actinomycetemcomitans Granulocyte medicine.disease biology.organism_classification Microbiology Respiratory burst medicine.anatomical_structure medicine Aggressive periodontitis Tannerella forsythia General Dentistry Porphyromonas gingivalis |
| Zdroj: | Oral Microbiology and Immunology. 24:124-132 |
| ISSN: | 0902-0055 |
| DOI: | 10.1111/j.1399-302x.2008.00484.x |
| Popis: | Introduction: Polymorphonuclear neutrophil (PMN) dysfunctions have been associated with severe forms of periodontitis. This study evaluated the correlation between PMN phagocytosis and oxidative burst with the subgingival microbiota of patients with generalized aggressive periodontitis (GAgP). Methods: Heparinized peripheral blood samples were obtained from 18 GAgP patients and 11 periodontally healthy (PH) subjects, and PMNs were isolated on a Ficoll–Hypaque gradient. For phagocytosis analysis, PMNs were incubated with fluorescein-labeled Staphylococcus aureus. The oxidative burst was evaluated by incubation of PMNs with dihydroethidium and activation by S. aureus. The assays were examined using flow cytometry. Subgingival biofilm samples were obtained from periodontal sites with and without periodontitis and 24 species were detected by checkerboard. Results: A significantly lower phagocytosis rate was observed for patients with GAgP compared with PH subjects over time (P |
| Databáze: | OpenAIRE |
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