| Autor: |
L. Hornok, A. Molnár, Maya Gasztonyi, Gyula Josepovits |
| Rok vydání: |
1987 |
| Předmět: |
|
| Zdroj: |
Pesticide Biochemistry and Physiology. 29:17-24 |
| ISSN: |
0048-3575 |
| DOI: |
10.1016/0048-3575(87)90080-0 |
| Popis: |
The binding of [ 14 C]carbendazim to tubulin-like and other soluble proteins was investigated in six strains of Fusarium oxysporum , two of them sensitive and four resistant to benomyl. The resistance in the latter ones was controlled by two different genes. The intact mycelium of the two resistant strains did not accumulate less fungicide from the incubation medium than a sensitive strain did. The fraction corresponding to the molecular weight of tubulin bound significantly less [ 14 C]carbendazim per milligram protein in the resistant strains than in the sensitive ones. Thus, the main biochemical feature of the resistant mutants studied here is their impaired carbendazim-binding activity. This alteration does not affect the site of action of phenylcarbamates. The decrease in binding of carbendazim is most pronounced in the strains possessing the ben1 mutation (8–12% of that in the sensitive strains). The binding of carbendazim to proteins of the whole extract was not lower in the resistant strains; moreover, it was significantly higher in ben2 mutants than in sensitive strains. This aspecific binding indicates that mutation at the ben2 locus affected not only tubulin but some other proteins as well. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
