Identification of anther-specific genes in a cruciferous model plant, Arabidopsis thaliana, by using a combination of Arabidopsis macroarray and mRNA derived from Brassica oleracea

Autor:	Katsunori Hatakeyama, Daisuke Shibata, Tohru Ariizumi, Kinya Toriyama, Masayuki Amagai, Makoto Endo, Masao Watanabe, Chikara Kuwata
Rok vydání:	2003
Předmět:	Genetics biology fungi food and beverages Brassicaceae Cell Biology Plant Science biology.organism_classification Genome Crucifer Complementary DNA Arabidopsis Arabidopsis thaliana Brassica oleracea Gene
Zdroj:	Sexual Plant Reproduction. 15:213-220
ISSN:	1432-2145 0934-0882
DOI:	10.1007/s00497-002-0159-z
Popis:	Arabidopsis thaliana, a member of the Brassicaceae, is a model plant whose genome was the first higher plant genome to be sequenced. Because of the small size of the flowers, it is difficult to dissect and separate reproductive organs (anthers and pistils) at different developmental stages in A. thaliana. In order to perform genome-wide identification of anther-specific genes in A. thaliana, an Arabidopsis cDNA macroarray was hybridized to cDNA derived from anthers and pistils of another crucifer, Brassica oleracea. After scanning the signal intensity for each clone, and cluster analysis, 52 anther-specific genes were identified. These clones contained several anther-specific genes that have already been characterized, as well as novel anther-specific genes. In RT-PCR analysis with mRNA of A. thaliana and B. oleracea, the expression pattern of one-third of the clones was similar to that determined by cDNA macroarray. This system of heterologous hybridization analysis (Arabidopsis cDNA macroarray vs Brassica tissue-specific mRNA) should be applicable to other model species and their close relatives.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::fba01dfede4e6d735860581121b0c055 https://doi.org/10.1007/s00497-002-0159-z Zobrazit plný text záznamu