Effect of continuous feeding of galactose on the production of recombinant glucose oxidase using Saccharomyces cerevisiae

Autor:	Young Hoon Park, Arnab Kapat, Joon Ki Jung
Rok vydání:	2000
Předmět:	biology Aspergillus niger Saccharomyces cerevisiae Bioengineering General Medicine biology.organism_classification Yeast law.invention chemistry.chemical_compound Biochemistry chemistry law Galactose biology.protein Bioreactor Recombinant DNA Fermentation Glucose oxidase Biotechnology
Zdroj:	Bioprocess Engineering. 23:37-40
ISSN:	1615-7591
DOI:	10.1007/s004499900117
Popis:	A recombinant strain of Saccharomyces cerevisiae harboring GOD gene originated from Aspergillus niger was used for the production of extracellular glucose oxidase. The effect of continuous galactose feeding on the induction of GAL-10 promoter was examined in a 5 l bioreactor. The highest enzyme production level (164 U cm−3) was achieved at 96 h of cultivation. The production performance was compared with the results of fed-batch cultivations carried out in the same laboratory. Continuous feeding mode was found to be less productive due to excess ethanol formation and plasmid instability.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::fb991b1fd3bf657351506a3d5484aa14 https://doi.org/10.1007/s004499900117 Zobrazit plný text záznamu Full text from SpringerLink