Two-photon ratiometric carbon dot-based probe for real-time intracellular pH monitoring in 3D environment
| Autor: | Zufu Lu, Pooria Lesani, Mohammad Mirkhalaf, Gurvinder Singh, Hala Zreiqat, Elizabeth J. New |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
General Chemical Engineering
Intracellular pH Nanoprobe General Chemistry Fluorescence Industrial and Manufacturing Engineering law.invention chemistry.chemical_compound chemistry Two-photon excitation microscopy Confocal microscopy law Extracellular Biophysics Environmental Chemistry Fluorescein Intracellular |
| Zdroj: | Chemical Engineering Journal. 433:133668 |
| ISSN: | 1385-8947 |
| DOI: | 10.1016/j.cej.2021.133668 |
| Popis: | Designing a two-photon ratiometric pH-sensitive nanoprobe for real time monitoring of intracellular pH in biological environment is of great importance for better understanding the pathogenesis of diseases and the design of intracellular drug delivery-based system. However, the development of such probe remains a challenge. Here, we report for the first time the design and development of the two-photon ratiometric carbon dot-based nanoprobe and demonstrate its capability to monitor and quantify the intracellular cytoplasmic pH value, in real time. The nanoprobe comprised of a fluorescent carbon dot functionalized with a pH-responsive fluorescein dye (FACD). With increasing pH, FACD exhibits a clear ratiometric change in the emission intensity ratio, with sensitivity across a wide pH range in both extracellular and intracellular compartments. FACD is non-toxic to adipose stem cells in cell imaging experimental conditions and exhibits remarkable thermal stability, photostability, and two-photon near-infrared excitation capability. Using real-time dual-channel two-photon confocal microscopy we demonstrate the great potential of FACD as an efficient nanoprobe with high accuracy for the intracellular sensing of pH in living adipose stem cells seeded on either cell-culture dishes or on a 3D printed bioactive scaffold. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect