Sequential intraparticle Förster resonance energy transfer for multi-wavelength bioimaging
| Autor: | Yuriy Bandera, Mary K. Burdette, Eric Zhang, Jessica L. Binder, Isabell Foulger, Jason P. Weick, Stephen H. Foulger, Haley W. Jones |
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| Rok vydání: | 2021 |
| Předmět: |
Materials science
Shell (structure) Nanoparticle 02 engineering and technology 021001 nanoscience & nanotechnology Photochemistry Methacrylate 01 natural sciences Electronic Optical and Magnetic Materials 010309 optics chemistry.chemical_compound Förster resonance energy transfer chemistry Covalent bond 0103 physical sciences Propargyl Rhodamine B 0210 nano-technology Luminescence |
| Zdroj: | Optical Materials Express. 11:1742 |
| ISSN: | 2159-3930 |
| Popis: | Core/shell nanoparticles composed of a silica core over which a propargyl methacrylate (PMA) shell was polymerized around were synthesized. To employ the shell coating, the surface of the silica nanoparticles (SiNPs) was modified with an alkene-terminated organometallic silane linker that allowed for the covalent attachment of a poly(propargyl methacrylate) (pPMA) shell. The alkyne groups resulting from the pPMA shell were utilized in copper(I)-catalyzed azide/alkyne cycloaddition (CuAAC) reactions to attach azide-modified Förster resonance energy transfer (FRET) pairs of naphthalimide (azNap), rhodamine B (azRhod), and silicon phthalocyanine (azSiPc) derivatives to the shell surface. The luminescence of the system was manipulated by the covalent attachment of one, two, or three of the fluorophores resulting in no energy transfer, one energy transfer, or two energy transfers, respectively. When all three fluorophores were attached to the core/shell particles, an excitation of azNap with a wavelength of 400 nm resulted in the sequential energy transfer between two FRET pairs and the sole emission of azSiPc at 670 nm. These particles may have applications as bioimaging probes as their luminescence is easily detected using fluorescence microscopy. |
| Databáze: | OpenAIRE |
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