Evaluation of Development of the Rat Uterus as a Toxicity Biomarker
| Autor: | Jorgelina Varayoud, Maria Mercedes Milesi, Marlise Guerrero Schimpf, Enrique H. Luque |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Cell growth Period (gene) Uterus Physiology Biology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure chemistry Toxicity Rat uterus medicine Biomarker (medicine) Xenobiotic Postnatal day 030217 neurology & neurosurgery |
| Zdroj: | Toxicity Assessment ISBN: 9781071610909 |
| DOI: | 10.1007/978-1-0716-1091-6_9 |
| Popis: | The developing uterus is highly sensitive to a brief exposure to different substances, in particular those with endocrine-disrupting activity. Thus, exposure to environmental, nutritional, chemical, and other xenobiotic factors affecting signaling events during critical organizational periods can alter the normal course of uterine development with lasting consequences. In this chapter, we provide an experimental protocol to evaluate the development of the rat uterus as a toxicity biomarker at two different developmental time points: (1) the neonatal period, on postnatal day (PND) 8, and (2) the prepubertal period, on PND21. In this experimental approach, we propose to assess: (1) uterine morphology and cytodifferentiation, (2) uterine cell proliferation, and (3) the expression of proteins involved in uterine organogenetic differentiation. All these morphological and molecular markers are useful tools to determine the consequences of exposure to toxicants with the potential to disrupt the uterine development. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|