The Combinations Chitosan-Pam 3 CSK 4 and Chitosan-Monophosphoryl Lipid A: Promising Immune-Enhancing Adjuvants for Anticaries Vaccine PAc
| Autor: | Yongli Bi, Jiantao Xu, Zhongfang Liu, Mingwen Fan, Lingkai Su, Ying Yang, Qingan Xu, Yuhong Li, Hua Tang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Saliva endocrine system diseases medicine.medical_treatment Immunology Monophosphoryl Lipid A macromolecular substances Biology Microbiology 03 medical and health sciences 0302 clinical medicine Immune system In vivo medicine Immunogenicity technology industry and agriculture Antibody titer 030206 dentistry equipment and supplies biology.organism_classification Streptococcus mutans carbohydrates (lipids) 030104 developmental biology Infectious Diseases Parasitology Adjuvant |
| Zdroj: | Infection and Immunity. 87 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.00651-19 |
| Popis: | We previously demonstrated that recombinant protein PAc could be administered as an anticaries vaccine. However, the relatively weak immunogenicity of PAc limits its application. In the present study, we investigated the effect of two adjuvant combinations of chitosan plus Pam3CSK4 (chitosan-Pam3CSK4) and of chitosan plus monophosphoryl lipid A (chitosan-MPL) in the immune responses to the PAc protein in vivo and in vitro PAc-chitosan-Pam3CSK4 or PAc-chitosan-MPL promoted significantly higher PAc-specific antibody titers in serum and saliva, inhibited Streptococcus mutans colonization onto the tooth surfaces, and endowed better protection effect with significantly less caries activities than PAc alone. Chitosan-Pam3CSK4 and chitosan-MPL showed no statistically significant differences. In conclusion, our study demonstrated that the chitosan-Pam3CSK4 and chitosan-MPL combinations are promising for anticaries vaccine development. |
| Databáze: | OpenAIRE |
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