FRET-based rational strategy for ratiometric detection of Cu2+ and live cell imaging
| Autor: | Tapas K. Kundu, Dhanasekaran Karthigeyan, Thimmaiah Govindaraju, Debabrata Maity |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
Chemistry Metal ions in aqueous solution Inorganic chemistry Metals and Alloys Hydrazone Condensed Matter Physics Photochemistry Acceptor Fluorescence Surfaces Coatings and Films Electronic Optical and Magnetic Materials Metal Rhodamine chemistry.chemical_compound Förster resonance energy transfer visual_art Materials Chemistry visual_art.visual_art_medium Electrical and Electronic Engineering Instrumentation Biosensor |
| Zdroj: | Sensors and Actuators B: Chemical. 176:831-837 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2012.09.071 |
| Popis: | We present chemosensors with coumarin as donor and rhodamine as acceptor for ratiometric fluorescent imaging of Cu 2+ based on metal ion induced FRET off-on mechanism. Two chemosensors coumarin–rhodamine hydrazone ( CRO ) and coumarin–rhodamine thiohydrazone ( CRS ) ratiometrically detects Cu 2+ in judiciously chosen aqueous buffer medium. Free ligands ( CRO and CRS ) emit green fluorescence and in presence of Cu 2+ the emission colour changed to red as spirolactam ring of rhodamine opens up upon complexation with metal ion. CRO and CRS can be used for successful detection of Cu 2+ in presence of other competing metal ions by employing ratiometric fluorescence property. Probes CRO and CRS undergo 1:1 stoichiometric complexation with Cu 2+ with the calculated association constants (log K a ) of 8.81 and 8.75 M -1 for [ CRO :Cu 2+ ] and [ CRS :Cu 2+ ] respectively. The probes CRO and CRS were employed as ratiometric biosensor for living cell imaging of Cu 2+ in HEK293T cells. |
| Databáze: | OpenAIRE |
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