Phenotypic and Genotypic Characteristics of Uropathogenic Escherichia coli Isolates from Kenya
| Autor: | Lillian Musila, Clement Masakhwe, Lilian Ogonda, Cecilia Kyanya, Daniel Matano, Catherine Wawira Muriuki, Erick Odoyo |
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| Rok vydání: | 2022 |
| Předmět: |
Microbiology (medical)
Pharmacology Phylogenetic tree medicine.drug_class Immunology Antibiotics Esbl production biochemical phenomena metabolism and nutrition Biology urologic and male genital diseases bacterial infections and mycoses medicine.disease_cause Microbiology Phenotype female genital diseases and pregnancy complications Antibiotic resistance Genotype polycyclic compounds medicine Cefoxitin Escherichia coli medicine.drug |
| Zdroj: | Microbial Drug Resistance. 28:31-38 |
| ISSN: | 1931-8448 1076-6294 |
| Popis: | Introduction: Uropathogenic Escherichia coli (UPECs) are a significant cause of urinary tract infections (UTIs). In Kenya, UTIs are typically treated with β-lactam antibiotics without antibiotic susceptibility testing, which could accelerate antibiotic resistance among UPEC strains. Aim: This study determined the occurrence of UPEC producing extended-spectrum β-lactamases (ESBLs), the genes conferring resistance to β-lactams, and the phylogenetic groups associated with ESBLs in Kenyan UPECs. Methodology: Ninety-five UPEC isolates from six Kenyan hospitals were tested for ESBL and plasmid-mediated AmpC β-lactamase (pAmpC) production by combined disk diffusion and disk approximation tests, respectively. Real-time and conventional polymerase chain reactions (PCRs) were used to detect three ESBL and six pAmpC genes, respectively, and phylogenetic groups were assigned by a quadruplex PCR method. Results: Twenty-four percent UPEC isolates were ESBL producers with blaCTX-M (95.6%), blaTEM (95.6%), and blaSHV (21.7%) genes detected. Sixteen isolates had blaCTX-M/TEM, whereas five had blaTEM/CTX-M/SHV. A total of 5/23 ESBLs were cefoxitin resistant, but no AmpC genes were detected. The UPECs belonged predominantly to phylogenetic groups B2 (31/95; 32.6%) and D (30/95; 31.6%), while groups B2 and A had the most ESBL producers. Conclusions: β-Lactam antibiotics have reduced utility for treating UTIs as a quarter of UPECs were ESBL producing. Single or multiple ESBL genes were present in UPECs, belonging primarily to phylogenetic groups B2 and A. |
| Databáze: | OpenAIRE |
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