N-trans-feruloyltyramine Protects Human Neuroblastoma SK-N-SH Cell Line Against H2O2-Induced Cytotoxicity
| Autor: | Rungtip Soi-ampornkul, Ei Ei Phyo Myint, Wipawan Thangnipon, Nutnicha Tantarungsee, Chalermchai Mitrpant, Patoomratana Tuchinda, Saksit Nobsathian, Chinnavuth Vatanashevanopakorn |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Natural Product Communications. 17:1934578X2211173 |
| ISSN: | 1555-9475 1934-578X |
| DOI: | 10.1177/1934578x221117312 |
| Popis: | Excessive accumulation of reactive oxygen species (ROS) has been regarded as a major contributor of pathogenesis in neurodegenerative diseases. N- trans-feruloyltyramine (NTF), an alkaloid isolated from several plants, has demonstrated an ability to be a potent antioxidant. In this study, the antioxidative and anti-apoptotic properties of NTF extracted from the stems of Polyalthia suberosa were investigated in the human neuroblastoma cell line SK-N-SH. NTF at concentrations ranging from 10 µM to 500 µM were not toxic to cells and reduced intracellular ROS levels significantly. Furthermore, pre-treatment of NTF significantly decreased H2O2-induced ROS generation and attenuated H2O2-mediated cytotoxicity. An increase in the expression of Bax and activated caspase-3 and reduction of Bcl-2 mediated by H2O2 was reversed by pre-treating the cells with 100 µM NTF. Likewise, NTF suppressed the increase of caspase-3 activity induced by H2O2. In conclusion, the findings reveal that NTF improves H2O2-induced intracellular ROS generation and decreases apoptosis. These protective effects of NTF could be useful for oxidative stress-related neurodegenerative conditions. |
| Databáze: | OpenAIRE |
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