Light-induced conformational changes and energy transfer in red fluorescent protein
| Autor: | Silvia Völker, F. Konz, S. Bonsma, J. Gallus, R. Purchase |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Chemistry
Energy transfer Biophysics Analytical chemistry General Chemistry Condensed Matter Physics Laser Photochemistry Biochemistry Fluorescence Atomic and Molecular Physics and Optics Fluorescence spectroscopy Green fluorescent protein law.invention law Spectral hole burning Irradiation Excitation |
| Zdroj: | Journal of Luminescence. 107:203-212 |
| ISSN: | 0022-2313 |
| Popis: | Reversible conformational changes have been photo-induced in the red fluorescent protein DsRed at low temperature by wavelength-selective laser irradiation. We have found two new fluorescent forms: a shifted-red (SR-) and a new green (G′-) form that absorb and emit, respectively, ∼14 nm to the red and ∼80 nm to the blue of the ‘mature’ red (R-) form present in an un-illuminated sample of DsRed. Further, we have identified the 0–0 transitions of the various forms by spectral hole burning and estimated their ground-state energy differences and barrier heights by means of temperature-dependent excitation and fluorescence spectroscopy between 1.6 and 295 K. We have also proven that ‘downhill’ energy transfer takes place between these forms within the tetrameric structure of DsRed. |
| Databáze: | OpenAIRE |
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