Popis: |
The interaction of quinhydrone with monosodium glutamate (MSG) in aqueous medium has been investigated using colorimetric method at different pH conditions. Quinhydrone is a mixture of quinone and hydroquinone. In acidic pH, hydroquinone is dominating and the hydroxyl group of hydroquinone interacts with the amino/carboxylic acid group of glutamate ion due to hydrogen bonds thus influences the solution. While in alkaline pH, the colour of the quinhydrone is brown due to benzoquinone being dominating component and can interact with amino group of glutamate ion thus resulting in the formation of di-anilido-quinone. This will lead to an increase of intensity of quinhydrone solution. Glutamate being amphoteric in nature, provides an option to examine it in both acidic and alkaline pH. The effectiveness of detection of MSG in alkaline medium is better due to its favourable interactions. |