| Popis: |
The results of complex chemical, physical parameters and biological effects of the time evolution of water and glucose/sucrose solutions characteristics irradiated on an M-30 micron (12.5 MeV, 1 MRad) are presented. The eight samples of liquids (solutions) with different compositions had been choosing for investigations. Water radiolysis products have a significant indirect effect on the structure of DNA in living organisms, which is comparable to their destruction under direct radiation impact. Given that terrestrial radiation is a permanent factor in the existence of biota, the study of kinetics and the peculiarities of chemical-structural transformations for irradiated water, aqueous solutions, and their effects on the living organisms is also important. Glucose/sucrose, which are important representatives of complex carbohydrates had been chosen in this study due to their widespread use in medicine and the food industry. This paper establishes the temporal regularities, both long- and short-term, on the transformation of chemical, physical properties, and biological activity of the mentioned above solutions. On the example of microorganisms from different phylogenetic origin ( Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa ), the biological action of irradiated solutions was determined, depending on the irradiation dose and the method of solution preparation: irradiated glucose/sucrose solutions and distilled water; irradiated dry glucose/sucrose in irradiated water; irradiated dry glucose/sucrose in irradiated water. It was found that the all irradiated samples exhibit a greater inhibitory effect on the strain of Pseudomonas aeruginosa compare to the other tested bacterial strains. It has been also shown that irradiated sucrose and glucose solutions introduced into suspensions of this pathogenic strain resulted in it complete elimination immediately after irradiation and 24 h after irradiation. Solution of dry irradiated glucose in irradiated water shows complete absence of growth immediately after irradiation and 5 × 10 1 CFU / ml 24 hours after irradiation, however, a similar sucrose solution does not show this effect. Less profound but still strong efficacy had been observed to tested strain of staphylococci, but not to E. coli commensal bacterial strain. Experiments of this kind were conducted for the first time, so they are of scientific and practical importance, both in the applied sciences and in medicine. |
