Optical methods of investigation of the protein Langmuir: Blodgett films

Autor:	V. V. Savransky, Alexander P. Savitsky, Ilya V. Alexeev, Oleg I. Lobanov, T. B. Dubrovsky, D. E. Akindinov
Rok vydání:	1994
Předmět:	Materials science Microscope law Picosecond Analytical chemistry Fluorescence microscope Laser Luminescence Langmuir–Blodgett film Fluorescence Photon counting law.invention
Zdroj:	SPIE Proceedings.
ISSN:	0277-786X
DOI:	10.1117/12.167471
Popis:	The structure of multilayer Langmuir-Blodgett (LB) films made of protein (Bovine serum albumine)-co-proporphyrin(III) conjugate was investigated by means of fluorescence microscope coupled with the time-correlated single photon counting registration system. The map of coproporphyrin fluorescence from the surface of protein LB-films was measured with picosecond time resolution and spatial resolution 15 micrometers . The sample area 240 X 240 micrometers was irradiated step by step by a focused beam of Nd:YAG laser with an accumulation time of 3 sec per point. There were two different kinds of samples: those with regular and those with irregular distribution of fluorescent labels over the sample.© (1994) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Databáze:	OpenAIRE
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